Bio-inputs · water · measurement

Method

We don’t guess what the soil is doing. A chromatography plate and the lab tubes show it — life, a blockage, an imbalance — and only then does management change. Look first, then measure, then decide: that way you depend neither on a trend nor on us.

Pfeiffer The plate, in red light A Pfeiffer chromatography disc under red light. Neither a pest test nor a certificate: a qualitative image of organic matter, minerals and biology, so you don’t intervene blind.
Germinating seeds, with roots of different length
Seed The root coating The coating covers the root. When it germinates, a larger root is the positive effect.
Six axes

How we work

Six steps, in this order. Skip the measurement and the rest is opinion. At the end the team makes, observes and adjusts without us.

Context

Soil, water, climate, economics and team. What can be done here, not in a generic slide deck.

Living fertility

Home-made bio-inputs, cover, living roots, diversity, animals where they fit, and balanced plant nutrition (less “kill the pest”, more healing the metabolism). Microbiology is the foundation: it turns organic matter and minerals into nutrients the plant can absorb.

Water that stays

Keyline and water management so regeneration does not dry out in the first drought. Harvesting water so biology has something to work with.

Autonomy

We train the team to make, observe and adjust. Less and less outside dependence.

Economics

Every step has to make business sense and hold up in the farm’s accounts.

Pfeiffer · Restrepo

Soil chromatography · Pfeiffer method

We use circular soil chromatography — a technique associated with Ehrenfried Pfeiffer and spread in Latin America by Jairo Restrepo Rivera (with Sebastião Pinheiro: Cromatografía: imágenes de vida y destrucción del suelo) — as a qualitative, visual reading of soil quality: how organic matter, minerality and biological activity relate in a pattern comparable over time.

Learning: Xavier learned this practice with Jairo Restrepo. True Regenerative has no commercial relationship with Restrepo and no endorsement from him; we cite the school we actually work with.

Reference — Book and training at La Mierda de Vaca · Cromatografía: imágenes de vida y destrucción del suelo (Jairo Restrepo Rivera & Sebastião Pinheiro) and the chromatographic analysis course. The book’s reference chromatograms are © their authors — we do not republish them here without permission.

True Regenerative soil chromatograms on a work table
Our chromatograms.

What it is for on the farm

  • A baseline before intervening (biofactory, amendments, management).
  • Visual tracking between seasons: the team sees the change, not just a lab PDF.
  • Independence: with hands-on training, you learn to run and read it yourself.

Limits: it is a qualitative tool for diagnosis and teaching. It does not replace quantitative analyses (organic matter, nutrients, metals, residues, etc.) or a carbon MRV against an external standard. At True Regenerative we combine it with lab work and field observation; we don’t sell invented “certificates” based on a chromatogram.

In projects such as the transition of the Tormantos vegetable farm, chromatography helped read a soil poor in microbiology, heavily mineralised and out of balance, and prioritise rebuilding living fertility alongside other documented measures.

Pfeiffer · CuCl₂

Sensitive crystallisation · Pfeiffer (CuCl₂)

Besides circular chromatography, we work with sensitive crystallisation with copper chloride (method developed by Ehrenfried Pfeiffer with Erika Sabarth): a qualitative image that helps attend to form and the perceived quality of extracts (soil, compost, juices / foods, according to protocol).

What we use it for: teaching and visual comparison over time; training the team’s eye alongside lab and field work. It is a qualitative diagnostic complement, not an ISO label.

Limits — Crystallisation is a real physical phenomenon; reading it as “vitality” needs judgement and training. It does not replace HPLC, microbial counts or quantitative analytics. We do not present it as conclusive proof of nutritional quality nor as a commercial certificate.

Trophobiosis

Plant nutrition · trophobiosis (Chaboussou)

We work plant nutrition as a management axis: not only soluble NPK, but mineral balance, soil biology and plant metabolism. In that line we use Francis Chaboussou’s frame (the theory of trophobiosis): many pests and diseases thrive when the sap offers a favourable “menu” — e.g. excess free amino acids from imbalances, stress or certain practices —; a well-nourished, metabolically balanced plant is less attractive. Chaboussou describes two states. In proteosynthesis, the plant builds amino acids and sugars into proteins and tissue, and resists better. In proteolysis, breakdown dominates: free amino acids and sugars remain in the sap, the food many insects and fungi are looking for.

In practice

  • Shifting from “kill the insect” to healing plant metabolism and the soil that supports it.
  • Looking at the whole: soil, trace elements, photosynthesis, irrigation, bio-inputs and management. No miracle product.
  • Prioritise interventions that reduce dependence on reactive chemicals when the diagnosis allows it.

Limits: trophobiosis is a very useful practical compass, not a single law. Climate, variety, natural enemies and inoculum pressure also matter. We don’t promise “zero pests”, nor do we replace the agronomic and regulatory criteria for each crop.

Next step

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